Highly-adherent cells can grow directly on the nanopatterned surfaces as shipped, but for certain cell types it may be beneficial to modify or coat the surface with a variety of extracellular matrix molecules (Collagen, Fibronectin, Gelatin, Matrigel, etc.). We also have a suggested concentration in our manual for protein coating of our substrates using some of these molecules. Before starting your experiments, make sure to check our manuals where you can find the details of the protocols for each protein coating.